Histologically an experiment(Huggins, 1931) has been peformed to elucidate the potential Of ectopic new tone formation from the transitional epithelium of urinary tract.
The purpose of the present investigation was to study in the cellular reaction in heterotopic osteogenesis following implantation of pericranium to sutcutanecus tissue and intramuscular region and to examine the morphology of these bone related to time.
The specimen taken from all experimental animals were fixed in 10% neutral formalin, embedded in paraffin wax, sectioned at a thickness of 5m, and stained with hematexylin-eosin for general histologic findings. Observation was made of morptological changes in the collagin fiber, capillary vessels, of osteoid tissue and asteoblst arrangement, and grossly observed thickness, arrangement and configuration of new bone.
The result are as follows ;
1. The new bone formation was better in subfutaneous implantation than intramuscular implantation
2. The configuration of new bone was superior in groups which were covered to silastic blockwith pericranium.
3. The inflammatory reaction was nearly subside within two weeks in B2 but C2 was prolong to pseudocapsule for Blatioa and severe inflammatry reaction to three weeks.
4. The configuration of new bone formation of B1 and Cl were visible to irregular direction and irregular thickness but B2 and C2 were regular form and thickness, and so these were possible to use in reconstructive surgery.
5. The pericranium of control group was severe adhesive to dura at three weeks and new bone formation that was occupying to iatrogenic skull defect.
The possibility of producing bone from a malleable soft tissue source could offer a solution to many reconstructive problems of head and neck merits further study.
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